Platelet Aggregation Ristocetin Cofactor
AggRAM®

Advanced Modular System for Platelet Aggregation Ristocetin Cofactor


AggRAM for platelet aggregationAggRAM

Cat. No. Item
1484 AggRAM Analyzer
1486 AggRAM Module

The AggRAM, for platelet aggregation and ristocetin cofactor testing offers unsurpassed precision, ease of use and flexibility. AggRAM couples four-channel laser optic modules with a powerful Windows interface. Up to two modules can be run per Remote Analyzer computer, providing up to eight channels to handle your workload.


Advanced Laser Optics

Unlike the tungsten lamp optics of old, the AggRAM uses a laser diode to provide beautiful precision across all channels. Upon start-up, AggRAM performs a laser balance check to ensure reproducibility day to day, year to year. Advanced optics coupled with an improved algorithm for slope calculation greatly improve precision for ristocetin cofactor testing.

calculates slope and max % aggregation
Powerful, User-Friendly Interface

The AggRAM system software takes full advantage of the Windows platform. On-screen prompts step users through testing. The ability to open multiple windows greatly increases efficiency. For instance, watch the channel display in real time in one window while starting a new worklist in another. Open a third window and print previous runs or observe any combination of channels. Additional features include:


Three levels of password protection, from user to administrator
Quick access to data input screens
Barcode and bi-directional LIS capable

Data Handling Features
Automatically calculates slope and max % aggregation; displays and print lag phase; automatically calculates curves and smooths; reference overlay

Customized Reports
Includes data from one channel or four, one patient or the whole run; pulls from data archives to profile patient results over time.

Quality Control
Evaluates and charts QC for ristocetin cofactor based on Westgard rules; generates Levy-Jennings charts, standard curves, etc with an integral action log.

 

 

 

platelet aggregation and Ristocetin CofactorAggRAM Specifications


Test Types: Platelet Aggregation and Ristocetin Cofactor
Absorbance Range: 0.0 to 2.0 O.D.
Measuring Wavelength: 650 nm
Optical Chambers per module: Four chambers for combined or individual measurements
Cuvettes: 8 mm x 60 mm (silicone coated glass)

 

Stir Bars: 3.5 mm x 4 mm (coated magnet)
Incubation and Reaction Temperature: 37°C +/-1°C
Graphs: -20% to 110% activity (+/- 0.5%) versus time
Instrument Operating Environment: Ambient Temperature Range 15° to 30°C (59° to 86°F)
Module:
Input Power: 110/220 Vac, 50/60 Hz, 1200 Watts Maximum
Dimensions: 6" (15.24 cm) Tall x 10" (25.40 cm) Wide x 17" (43.18 cm) Deep
Weight: < 15 lbs (6.75 kg)
Computer:
800 MHz or higher processor (Intel Pentium/Celeron family)
64 MB or higher of RAM
1.5 GB of available hard disk space
Microsoft® Windows® 2000 Operating System
CD-RW drive
Super VGA (800 x 600) or higher resolution video adapter monitor.
Keyboard and Mouse.
Hp Deskjet 6122 Printer.

AggRAM Accessories and Supplies

Cat. No. Item
1473 Siliconized Cuvettes for AggRAM and PACKS-4, 200/pkg
1479 Scale Set Solutions (650 nm) for Platelet Assays, 1 each Low and High Calibrator
1489 AggRAM Stir Bars, 30/pkg
9139 Service Manual for AggRAM

 

Platelet Aggregation Reagents

Cat. No. Item
5366 ADP, 2 x 1 mL
5367 Epinephrine, 2 x 1 mL
5368 Collagen, 2 x 1 mL
5369 Platelet Aggregation Kit (5366, 5367, 5368)
5199 Ristocetin (for RIPA), 10 x 0.5 mL
5365 Tris-Buffered Saline, 1 x 125 mL

 

Ristocetin Cofactor Reagents

Cat. No. Item
5370 Ristocetin Cofactor Kit, 60 tests
5371 Lyophilized Platelets, 5 x 5 mL
5373 Risto Cofactor Abn Control, 5 x 0.5 mL
5372 Ristocetin (10 mg/mL), 5 x 1.5 mL
5365 Tris-buffered Saline, 1 x 125 mL
5356 Lyophilized Platelets, 5 x 10 mL

 


PACKS-4 Accessories and Replacement Parts

Cat. No. Item
1463 Air Filters for PACKS-4, 3/pkg
1473 Siliconized Cuvettes for AggRAM and PACKS-4, 200/pkg
1474 PACKS-4 Stir Bars, 30/pkg
1475 Disposable Pipette Tips (50-200 µL), 1000/pkg
1479 Scale Set Solutions (650 nm) for Platelet Assays, 1 each Low and High Calibrator


Platelet Aggregation Reagents

platelet aggregation kitPlatelet Aggregation Kit

Cat. No. Item
5369 Platelet Aggregation Kit
  ADP Reagent, 2 x 1 mL
  Collagen Reagent, 2 x 1 mL
  Epinephrine Reagent, 2 x 1 mL

The Platelet Aggregation Kit is used to identify normal and abnormal platelet function, to quantitate platelet response and to determine the patient's response to anti-platelet drug therapy. The reagents are optimized for use with the Helena AggRAM analyzer, but may be used on any turbidometric aggregometer of similar quality.

ADP reagentADP Reagent

Cat. No. Item
5366 ADP, 2 x 1 mL

ADP is a lyophilized preparation of adenosine-5-diphosphate. ADP is for use in routine platelet aggregation studies for the evaluation of qualitative platelet function disorders. The concentration of the reconstituted ADP reagent is 200 mM.

 

collagen reagentCollagen Reagent

Cat. No. Item
5368 Collagen, 2 x 1 mL

The collagen reagent is a clear, stabilized liquid reagent prepared from equine tendon, which is one of the world's best collagen preparations. Collagen is used in routine platelet aggregation studies for evaluation of qualitative platelet function disorders. The collagen reagent stock solution is reconstituted to a concentration of 100 µg/mL.


epinephrine reagentEpinephrine Reagent

Cat. No. Item
5367 Epinephrine, 2 x 1 mL

Epinephrine is a lyophilized preparation of L-epinephrine bitartrate. Epinephrine is for use in routine platelet aggregation studies for evaluation of qualitative platelet function disorders. After reconstitution, epinephrine concentration is 3 mM.


arachidonic acid reagentArachidonic Acid Reagent

Cat. No. Item
5364 Arachidonic Acid, 2 x 1 mL

Arachidonic Acid is a lyophilized preparation of sodium arachidonate for use in platelet aggregation studies of abnormal function, especially dysfunction induced by aspirin and aspirin-containing medications. The concentration of the reconstituted reagent is 5 mg/mL.

 

ristocetin for aggregation assaysRistocetin (for Aggregation Assays)

Cat. No. Item
5199 Ristocetin, 10 x 0.5 mL

This Ristocetin is for use in platelet aggregation studies for confirmation of von Willebrand Disease (vWD) and Bernard-Soulier Syndrome. Normal platelet-rich plasma exhibits a positive aggregation response to the addition of ristocetin using a final concentration of 12-15 mg/mL. The majority of vWD patients exhibit a negative response to ristocetin as do Bernard-Soulier patients.

ristocetin cofactor assay kitHelena Ristocetin Cofactor Assay Kit

Cat. No. Item
5370 Ristocetin Cofactor Kit, 50 tests
  Lyophilized Platelets, 4 x 5.0 mL
  Ristocetin Cofactor Abnormal Control, 2 x 0.5 mL
  S.A.R.P. Assayed Reference Plasma, 2 x 1.0 mL
  Helena Ristocetin - 10 mg/mL, 2 x 1.5 mL
  Tris-Buffered Saline, 1 x 35 mL

Ristocetin cofactor assays measure the ability of von Willebrand factor (Ristocetin cofactor) in the patient's plasma to agglutinate formalinized platelets in the presence of ristocetin. Decreased levels of ristocetin cofactor activity are associated with von Willebrand Disease.

 

lyophilized plateletsLyophilized Platelets

Cat. No. Item
5356 Lyophilized Platelets, 5 x 10 mL
5371 Lyophilized Platelets, 5 x  5 mL

Lyophilized Platelets are a standardized suspension of fixed platelets derived from platelet concentrate. Lyophilized Platelets are for use in the quantitation of ristocetin cofactor activity in plasma. The platelets are lyophilized for stability. Tris-Buffered Saline (Cat. No. 5365) should be used for reconstitution. The concentration of the reconstituted platelet suspension is approximately 200,000/mL. Platelets in Cat. No. 5356 show a slower reaction rate.


Ristocetin Cofactor Abnormal Control PlasmaRistocetin Cofactor Abnormal Control Plasma

Cat. No. Item
5373 Ristocetin Cofactor Abnormal Control, 5 x 0.5 mL


The control is to be used in the assay of ristocetin cofactor. It contains citrated human plasma with a level of ristocetin cofactor activity below 50%.


Ristocetin Cofactor AssaysRistocetin - 10 mg/mL (for Ristocetin Cofactor Assays)

Cat. No. Item
5372 Ristocetin - 10 mg/mL, 5 x 1.5 mL


The reagent is to be used in the quantitative determination of ristocetin cofactor activity. The ristocetin reagent is prepared from Nocardia lurida and is used in the tests for von Willebrand Disease and Bernard-Soulier Syndrome.

 

Tris-Buffered SalineTris-Buffered Saline

Cat. No. Item
5365 Tris-Buffered Saline, 1 x 125 mL


Tris-Buffered Saline is used for reconstitution and dilution of some of the reagents used in platelet aggregation assays and ristocetin cofactor assays.

 



Platelet Aggregation

Platelet Aggregation Reagents

Helena's platelet reagents provide outstanding stability and performance at affordable pricing. Reagents are optimized for use with AggRAM, but may be used on any turbidometric aggregometer of similar quality.

Ristocetin Cofactor

Ristocetin Cofactor Reagents

Ristocetin Cofactor is more sensitive than RIPA to mild reductions in vWF and more often used to monitor therapy in vWD patients. Kit includes ristocetin, formalinized platelets, S.A.R.P., abnormal control and tris-buffered saline.

AggRAM Brochure