Electrophoresis Supplies, Power Supplies, and Blotters

Helena catalog

Electrophoresis Power Supplies

TITAN® Plus Power Supply

Cat. No.	Item
1504	TITAN Plus Power Supply, 110V
1505	TITAN Plus Power Supply, 220V

TITAN Plus is a completely solid state unit. Digital voltage and current display provides greater accuracy in voltage selection. Synchronous electrophoresis timer assures precise control; timer automatically shuts off current and signals end of run. For use with cellulose acetate, agarose and agar. Recommended for use with Quick Gel Chamber, (Cat. No. 1284).

Titan Plus Specifications
Voltage: 0-500 VDC. Digital display. Accuracy ± 3V.
Current: Up to 125 mA. Digital display. Accuracy ± 2 mA.
Chamber Requirements: Accepts all standard electrophoresis chambers
Timer: Synchronous, electrical. Up to 60 minutes automatic timing. Audiovisual signal indicates end of run. "Hold" allows for continuous operation beyond 60 minutes.
Safety Feature: Fuse holder: 110V-1A; 220V-.5A, 3 prong plug, grounded chassis
Output Jacks: Two sets labeled three ways: + and -, Red and Black; Positive and Negative. Standard banana jack spacing.
Dimensions: 5" H x 10" W x 9.5" D (12.8 x 25.5 x 24.2 cm)
Weight: 11 lbs. (4.95 kg)

EWS Digital Power Supply

Cat. No.	Item
1520	EWS Power Supply, 110V
1521	EWS Power Supply, 220V
1150	Comprehensive Replacement Agreement for EWS

The microprocessor-controlled EWS Power Supply provides a digital display showing voltage/current levels with ± 1% accuracy and elapsed time ± second. Timer allows programming in 1 second intervals up tp 99 minutes, 59 seconds. Power output ranges from 50-400 VDC in constant voltage mode in 1.0 V increments; 2-60 mA in constant current mode in 0.1 mA increments. Standard banana plug output. Recommended for use with Laurell Rocket electrophoresis procedures (Cat. Nos. 5357 and 5359).

EWS Specifications
Modes of Operation: Constant voltage or constant current. Time automatically or manually controlled.
Input Power: Constant Voltage Output Range: 50 to 400 VDC ± 1% or ± 1 unit in 1.0V increments. Constant Current Output: 2 to 60 mA ± 1% or ± 1 unit in 0.1 mA increments.
Meter Accuracy: ± 1V or ± 0.1 mA or ± 1%
Ripple (Noise): Less than 1% rms of output amplitude
Timer: Up to 99 min. 59 sec. in 1 sec. increments
AC Fuse (Top): 2 A, 250V
High Voltage Fuse (Bottom): 0.2 A, 250V
Dimensions: 5" H x 10.2" W x 12" D (12.5 x 25.5 x 30 cm)
Weight: 5.5 lbs. (2.5 kg)

Electrophoresis Chambers and Accessories

TITAN GEL® Chamber

Cat. No.	Item
4063	TITAN GEL Chamber w/ Heat Shield, 1/pkg

The TITAN GEL Chamber is for Titan III cellulose acetate, TITAN GEL Agarose, Titan IV citrate agar and IEP gels. Disposable paper wicks or sponges allow use with electrophoresis media supported by a rigid, flat base up to 6" x 9". A heat shield is included to protect gels from heat buildup. Unique interlock system breaks electrical circuit when chamber lid is removed.

EWC Electrophoresis Chambers

Cat. No.	Item
1557	EWC Replacement Chamber

The EWC Replacement Chamber is a direct replacement part for the EWC Electrophoresis Chamber. Optimum results for high resolution protein electrophoresis require use of the TITAN GEL Chamber. The TITAN GEL Chamber for the EWC is equipped with a special plug for the EWC power supply.

Paper and Sponge Wicks

Cat. No.	Item
5081	Zip Zone Chamber Wicks, 500/pkg
9014	Sponge Wicks - Short, 4 short sponges/pkg
9015	Sponge Wicks - Long, 2 long sponges/pkg

Zip Zone Chamber Wicks are disposable paper wicks used with the Titan III cellulose acetate system to provide buffer flow during electrophoresis. Sponge Wicks provide sufficient buffer flow during agar and agarose techniques such as IEP and citrate hemoglobin electrophoresis.

Chamber Cooling Device and Coolant Sponges

Cat. No.	Item
3039	TITAN GEL Chamber Cooling Device, 1/pkg
5045	Coolant Sponges, 10/pkg

Cooling Device is used in TITAN GEL or Zip Zone chamber to reduce heat during TITAN GEL High Resolution Protein electrophoresis. The device is refrigerated, then placed in chamber immediately prior to electrophoresis. Gels are placed directly on cooling device and wicked to outside buffer troughs. Coolant sponges keep chambers cool during separations where heat can cause denaturation. Store sponges in freezer, then place in center troughs of chamber during electrophoresis.

Blotters

Titan® Blotter Pads

Cat. No.	Item
5034	Blotter Pads, 76 x 102 mm, 100/box
5037	Blotter Pads, 89 x 108 mm, 100/box
5098	Blotter Pads, 203 x 241 mm, 25/pkg

These soft, absorbent paper blotters are used to remove excess buffer or reagent from cellulose acetate membranes or agarose gels.

Note: Blotters for Helena agarose procedures are packaged in each gel kit.

Electrophoresis Buffers

Electra®, Citrate and Supre-Heme® Buffers

Cat. No.	Item
5805	Electra® HR Buffer Tris-Barbital/Sodium Barbital, 10 x 18 g
5016	Electra® B₁ Buffer Barbital/Sodium Barbital/Na Azide, ... 10 x 13.1 g
5121	Citrate Buffer Sodium Citrate/Citric Acid, 10 x 15.5 g
5802	Supre-Heme® Buffer Tris-EDTA/Boric Acid, 10 x 14.6 g
Note: Appropriate buffers for SPIFE, REP and TITAN GEL Agarose kits are packaged as part of each kit.

Stains, Clearing Solutions

Cat. No.	Item
3048	Amido Black Protein Stain, 1 x 2.5 g

Used as an alternative to Coomassie Brilliant Blue in the TITAN GEL High Res Protein procedure and for other procedures requiring a good basic protein stain. Dissolve stain in 450 mL methanol, 450 mL deionized water and 100 mL glacial acetic acid.

3049

TITAN GEL IEP Stain, 1 x 10 g

Coomassie Brilliant Blue stain for use with the TITAN GEL IEPlate Kit (Cat. No. 3047). Dissolve in 450 mL ethanol, 450 mL deionized water and 100 mL glacial acetic acid.

5322	Lipoprotein Stain, 1 x 2.8 g
Fat Red 7B stain for use with Titan III-Lipo cellulose acetate. Dissolve stain in 1000 mL methanol.

5036	o-Dianisidine, 1 x 2 g
Hemoglobin-specific stain for Titan IV Citrate Agar. Dissolve stain in 1000 mL methanol.

5041	o-Tolidine, 1 x 2 g
Hemoglobin-specific stain yields blue-colored bands with Titan IV Citrate Agar. Not recommended when 5% acetic acid wash is used. Dissolve stain in 1000 mL methanol.

5526	Ponceau S Stain, 1 x 105 g
Multi-purpose protein stain, especially useful for serum proteins and alkaline hemoglobins on cellulose acetate. Dissolve in 1000 mL deionized water.

5362	Rocket Stain, 1 x 0.9 g
Coomassie Brilliant Blue stain used with Helena Rocket plates for Proteins C and S, vWF:Ag. Dissolve stain in 450 mL methanol, 450 mL deionized water and 100 mL glacial acetic acid.

5005	Clear Aid, 1 x 250 mL
Clearing solution additive that prevents cellulose acetate from shrinking during the clearing step. Add this high molecular weight elastomer as directed in the cellulose acetate procedures for proteins and alkaline hemoglobins.

4950	PermaClear, 1 x 1 L
A substitute for the clearing solution prepared with Clear Aid. Not recommended for use with AVES or AVES 2. Dilute PermaClear with deionized water (55 mL PermaClear/45 mL water).

Buffer and Stain Accessories

Bufferizer

Cat. No.	Item
5093	Bufferizer, 1/pkg
5094	Bufferizer Bottoms w/ Lids, 3/pkg
Bufferizer is used to uniformly soak cellulose acetate membranes prior to electrophoresis. To use, place up to 12 dry membranes in a carrying rack; set rack in Bufferizer bottom; place Bufferizer top over bottom; pour buffer into top. Buffer gradually drips through a small hole in the top, allowing a uniform liquid front to move up the dry membranes. Eliminates air bubbles caused by uneven soaking.

Staining Sets and Carrying Racks

Cat. No.	Item
5122	1000 mL Staining Set, 7 trays with lids, 1 rack
5110	Titan III Carrying Rack, 1/pkg

Staining set consists of 7 plastic trays with lids and a carrying rack for staining and soaking of cellulose acetate membranes. Trays hold 1000 mL of liquid. Carrying rack is designed to hold up to twelve 60 x 76 mm or 94 x 76 mm Titan III cellulose acetate membranes. One rack is included with the staining set; rack is also available separately.

Staining Dishes

Cat. No.	Item
4061	Staining Dish, 1/pkg
1362	REP Gel Staining Dish, 10/pkg

The Lipoprotein Staining Dish is 175 x 102 x 50 mm and may be used for cellulose acetate and agarose lipoprotein electrophoresis procedures. The REP Gel Staining Dish is 216 x 168 x 19 mm and may be used for REP procedures requiring staining.

Stain Dish Set and Racks

Cat. No.	Item
1558	TITAN GEL Multi-Staining Set, 4 stain dishes, 3 racks, 1 base
1555	TITAN GEL Stain Rack for 8- or 10-sample gels

TITAN GEL Multi-Staining Set provides additional stain dishes and racks for labs requiring multiple stain setups.

Incubation Chamber and Development Weight

Incubation Chamber

Cat. No.	Item
4062	TITAN GEL Isoenzyme Incubation Chamber, 1/pkg

The TITAN GEL Isoenzyme Incubation Chamber is 171 x 89 x 6 mm and holds one gel. It maintains adequate humidity during the incubation process when running TITAN GEL CK and LD isoenzyme and ImmunoFix procedures.

Development Weight

Cat. No.	Item
5014	Development Weight, 102 x 108 mm, 1/pkg

The Development Weight can be used to press agarose gels. The weight also provides a constant surface temperature for incubating cellulose acetate membranes. Membranes are sandwiched between a prewarmed weight and a glass slide, then placed in incubator.

Electrophoresis Supplies

Micropreparation Dish

Cat. No.	Item
8003	Micropreparation Dish, 1/pkg

The micropreparation dish is convenient for preparing samples for hemoglobin procedures and fetal lung maturity testing by TLC. It is constructed of chemically resistant nylon. Eight separate sample areas accommodate volumes of 1 mL or less. The conical shape assures easy access to entire sample solution.

Titan® and TITAN GEL® Plastic Envelopes

Cat. No.	Item
5052	Titan Envelopes, 121 x 64 mm, 200/pkg
5053	Titan Envelopes, 115 x 102 mm, 100/pkg
5054	TITAN GEL Envelopes, 86 x 156 mm, 100/pkg

These clear plastic envelopes, with convenient overlapping sleeve, enable you to store completed electrophoresis membranes and gels without scratching or tearing. They afford ideal protection for both completed TITAN III cellulose acetate and TITAN GEL agarose gels. Cat. No. 5054 can be used with Iso·Dot and Multi-Slot gels.

Identification Labels

Cat. No.	Item
5006	8-Sample Titan ID Labels, 500/pkg
5015	12-Sample Titan ID Labels, 400/pkg

Titan ID Labels are convenient, pressure-sensitive identification labels for Titan III membranes. Simply press label onto edge of membrane or Titan Plastic Envelope to provide a paper surface on which to identify the sample.

Helena® Marker

Cat. No.	Item
5000	Helena Marker, 1/pkg

The Helena Marker can be used to write identifying information on the support side of cellulose acetate membranes and agarose gels. Will not wash off in most solvents.

Helena Glue Stick

Cat. No.	Item
5002	Glue Stick, 1/pkg

The Glue Stick is an easy-to-use adhesive for mounting densitometric tracings on the report forms.

AVES Accessories and Replacement Parts

AVES Plate Holders and Racks

Cat. No.	Item
1297	AVES Slide Staining Rack

Plate holders hold up to three Titan III cellulose acetate membranes for electrophoresis, staining, clearing, and drying. The slide staining rack is made of plastic and holds up to 32 microscope slides for using AVES as a slide stainer.

AVES Electrophoresis Chamber and Sponges

Cat. No.	Item
1299	AVES Electrophoresis Chamber, 1/pkg
1294	AVES Replacement Sponges, 4 pairs/pkg

The AVES Electrophoresis Chamber may be purchased as a replacement for the original chamber on the unit. The sponges are replacement sponge wicks for the upper and lower electrodes of the AVES Electrophoresis Chamber.

REP Preparation Solutions, REP Prepper

Cat. No.	Item	Description
1574	SUREprep	3 x 250 mL
3100	REP Prep Solution	1 x 250 mL
1359	REP Prepper	1/pkg

SUREprep is a priming agent for REP applicator tips, ensuring precise sample application. It's also a cleaning agent for the tips. REP Prep is a wetting agent applied to the floor of the electrophoresis chamber to promote complete contact between gel and chamber floor. The dispenser bottles make it easy to apply SUREprep and deionized water; packaged to provide one dispenser each for SUREprep and deionized water. REP Prepper is a device for air-drying the pre-cut sample wells in REP gels. Sample wells must be free of moisture when samples are applied in order to maintain precise sample absorption.

REP and REP 3 Accessories and Replacement Parts

Cat. No.	Item	Description
1044	DZA Replacement Lamp for REP (not for REP 3)	1/pkg
1117	UV Replacement Lamp	1/pkg
1361	REP/SPIFE Contact Sheets	5/pkg
1362	REP Gel Staining Dish	10/pkg
1364	Replacement REP Pipette Assembly
1577	Replacement Tips for REP	17/pkg

REP and REP 3 Electrodes, Reagent Spreaders (Not interchangeable between REP and REP 3)
Cat. No.	Item	Description
1349	REP IEF (Isoelectric Focusing) Electrodes & Adapters	3 electrodes, 2 adapters
1360	REP Electrodes	2/pkg
1363	REP Glass Rods (for ALP, LD, HDL)	2/pkg
3387	Applicator Blade Weights (for SPIFE, REP)	3/pkg
3706	Reagent Spreaders for SPIFE 3000 and REP 3	2/pkg
3704	SPIFE/REP 3 IFE Electrodes & Adapters	3 electrodes, 2 adapters
3709	SPIFE/REP 3 Replacement Electrodes	2/pkg

Printer Supplies and Diskettes for REP Analyzers
Cat. No.	Item	Description
1424	Toner Cartridge for Brother 1240 or 1440 Laser Printer	1/pkg
1425	Drum for Brother 1240 Laser Printer	1/pkg
1465	Toner Cartridge for Brother HL-630 Laser Printer	1/pkg