Hemostasis, Microplate Reader and Washer, ELISA Assays

ELISA Assays

von Willebrand Factor Antigen (vWF:Ag) by ELISA

Cat. No.

Item

5290

vWF:Ag ELISA Kit, 96 tests
vWF:Ag Microwell Plate, 12 x 8-well strips

Sample Diluent, 1 x 60 mL

ELISA Reference Plasma, 3 x 0.5 mL

vWF:Ag Conjugate Solution, 1 x 12 mL

Substrate, 1 x 13 mL

Stopping Solution, 1 x 15 mL

Phosphate Buffered Saline Concentrate, 1 x 30 mL

This vWF:Ag assay uses an ELISA antibody capture sandwich technique to quantitate vWF:Ag in human plasma and is standardized against established reference preparations. Assay time is about an hour and final results are read at 450 nm. The key advantages of this new ELISA assay are its fast turnaround time and its ease of use. vWF:Ag is important in evaluating the pro-coagulant system, and von Willebrand Disease in particular.

Protein C Antigen by ELISA

Cat. No.

Item

5291

Protein C ELISA Kit, 96 tests

Protein C Microwell Plate, 12 x 8-well strips

Sample Diluent, 1 x 60 mL

ELISA Reference Plasma, 3 x 0.5 mL

Protein C Conjugate Solution, 1 x 12 mL

Substrate Solution, 1 x 13 mL

Stopping Solution, 1 x 15 mL

Phosphate Buffered Saline, 1 x 30 mL

This Protein C assay uses an antibody capture sandwich technique to quantitate Protein C in human plasma. Quantitative deficiencies of Protein C are associated with an increased tendency for thrombosis. The Protein C ELISA assay can be completed in about 90 minutes and is read at 450 nm. Its fast turnaround time and ease of use offer advantages over other Protein C assays.

Free and Total Protein S Antigen by ELISA

Cat. No.

Item

5292

Protein S (Free & Total) ELISA Kit, 96 tests
Protein S Microwell Plate, 12 x 8-well strips

Sample Diluent, 1 x 60 mL

Free Protein S Reagent (PEG), 1 x 2 mL

ELISA Reference Plasma, 3 x 0.5 mL

Protein S Conjugate Solution, 1 x 12 mL

Substrate Solution, 1 x 13 mL

Stopping Solution, 1 x 15 mL

Phosphate Buffered Saline, 1 x 30 mL

This 90-minute ELISA assay for quantitation of free and total Protein S in human plasma is easy to perform and offers a rapid turnaround time. The assay is read at 450 nm. To determine free Protein S, the physiologically active form, the sample is pretreated with PEG to precipitate the C4b-binding protein complex. Deficiencies of Protein S are associated with increased thrombosis.

Monoclonal Free Protein S Antigen by ELISA

Cat. No.

Item

5293

Monoclonal Free Protein S ELISA Kit, 96 tests

Free Protein S Microwell Plate, 12 x 8-well strips

Sample Diluent, 1 x 60 mL

ELISA Reference Plasma, 3 x 0.5 mL

Protein S Conjugate Solution, 1 x 12 mL

Substrate Solution, 1 x 13 mL

Stopping Solution, 1 x 13 mL

Phosphate Buffered Saline, 1 x 30 mL

This assay uses a monoclonal antibody specific for Free Protein S in an ELISA format to measure Free Protein S directly without PEG precipitation. Incubation times for this procedure total 1 hour, allowing timely completion of testing. Results are read at 450 nm and calculated by comparison to an internationally recognized reference plasma.