Column Chromatography, Hemoglobin A2

Helena catalog

Hemoglobin A2 Quik Column Assays

Beta-Thal HbA2 Quik Column Kit

Cat. No.	Item
5341	Beta-Thal HbA2 Quik Column Kit, 50 tests 50 HbA2 Quik Columns 1 x 130 mL HbA2 Developer 1 x 20 mL Hemolysate Reagent-C

The Beta-Thal HbA2 Quik Column method is an anion exchange chromatography method that provides a rapid, accurate method for quantitating HbA2 and requires no specialized training or instrumentation. Total assay time is about 40 minutes.

Sickle-Thal Quik Column Kit

Cat. No.

Item

5334

Sickle-Thal Quik Column Kit, 25 tests
25 Sickle-Thal Quik Columns

1 x 130 mL HbA2 Developer

1 x 300 mL HbS Developer

1 x 20 mL Hemolysate Reagent-C

The Helena Sickle-Thal Quik Column Method is designed to quantitate HbA2 in blood samples from individuals with Sickle Cell Trait. The Helena Sickle-Thal Quik Column allows elution of HbA2 while HbA, HbS and HbF and many other abnormal hemoglobins are retained on the column. HbA2 may also be quantitated in blood samples containing no abnormal hemoglobins. HbS may be eluted for quantitation if desired.

HbA2 Quik Column Controls

Cat. No.	Item
5339	HbA2 Quik Column Control-Normal, 5 x 1 mL
5333	HbA2 Quik Column Control-Abnormal, 5 x 1 mL

The controls are used as quantitative controls for normal and elevated levels of HbA2 assayed by column chromatography. Controls are prepared from fresh, packed human erythrocytes. Reconstituted controls are stable 2 weeks stored at 2 to 6°C.