SPIFE IgG IEF

The SPIFE IgG IEF kit is intended for the identification of IgG-specific oligoclonal banding in serum and CSF using isoelectric focusing and immunoblotting. This technique is considered the “Gold Standard” method for the determination of intrathecal IgG synthesis in the clinical diagnosis of multiple sclerosis.  

When there is a restricted humoral immune response, oligoclonal bands can be detected in the CSF. The concentration of individual bands may be as low as 2% of the total CSF IgG but can be seen against the background of polyclonal IgG present through filtration from the blood. Due to this filtration, it is important to rule out oligoclonal IgG from the blood by running parallel serum samples against CSF samples. The presence of oligoclonal banding in CSF but not in serum is considered indicative of MS. With IEF, oligoclonal banding may be detected while the total CSF IgG concentration is still within the reference range. Isoelectric focusing is considered twice as sensitive as the IgG index and gives superior resolution over standard electrophoresis.

Advantages of SPIFE IgG IEF

• Simple procedure
• Performed on unconcentrated CSF
• Single-antibody IgG immunodetection
• Most sensitive procedure for humoral immune responses    

SPIFE IgG Isoelectric Focusing
for CSF and Serum

Kits include: Anode Solution, Cathode Solution, Blocking Agent, Acetate Buffer Concentrate, Chromogen, IgG IEF Antibody, Transfer Membranes, Blotters, Wicks, Sample Templates.

* Precut sample wells accommodate 5 profiles per gel; alternate template procedure allows 8 profiles per gel.

Additional Equipment Needed: SPIFE IgG IEF Electrodes (2/pkg) (Cat. No. 3383).