TITAN III Cellulose Acetate Alkaline Phosphatase Isoenzymes

Helena catalog

Titan® III Alkaline Phosphatase Isoenzymes

Alkaline phosphatase (ALP) in human serum originates from several tissue sources with the greatest activity resulting from bone, liver, intestine and placenta. Because of the wide distribution, limited information can be obtained from a total ALP assay. Fortunately the tissue sources of elevated ALP in serum can be determined by identifying the isoenzyme. ALP isoenzymes differ in their physicochemical and electrophoretic properties, and by taking advantage of these differences, individual isoenzymes can be identified. In addition to liver, bone, intestinal and placental isoenzymes, Regan, PA, Nagao and renal isoenzymes have also been identified.

Titan III Cellulose Acetate ALP Procedure Total Time: approximately 50 minutes.

Alkaline Phosphatase Indolyl Blue Reagents

Cat. No.

Item

5102

ALP Indolyl Blue Reagent

10 x 3 mL Reagent

1 x 30 mL Diluent

This reagent is for colorimetric development of ALP isoenzyme bands after electrophoresis. Results obtained with Indolyl blue are easily visualized and interpreted due to the uniform white background and bright intense bands. It can be used instead of the traditional reagents listed below.

Alkaline Phosphatase Flur/Vis Reagents

Cat. No.	Item
5119	ALP Flur-Vis Reagent Set 10 x 2 mL (ASMX) 10 x 2 mL (Fast Blue RR)
5118	ALP Isoenzyme Vis Reagent, 10 x 2 mL (Fast Blue RR)
5120	ALP Isoenzyme Flur Reagent, 10 x 2 mL (ASMX)

After electrophoresis, ALP isoenzymes can be developed fluorometrically or colorimetrically. Incubation times and techniques are identical. Visible detection requires both ALP Flur Reagent (ASMX) and Vis Reagent (Fast Blue RR).

Alkaline Phosphatase Isoenzyme Control

Cat. No.	Item
5139	ALP Isoenzyme Control, 1 x 1 mL

The control contains liver and intestinal alkaline phosphatase isoenzymes. It is prepared from tissue homogenates and is for qualitative and quantitative use with the Titan III cellulose acetate system. The control is a stabilized liquid.

Titan III Cellulose Acetate and HR Buffer for ALP Isoenzymes
Cat. No.	Item
3000	Titan III Iso-Vis Cellulose Acetate, 60 x 76 mm
3001	Titan III Iso-Vis Cellulose Acetate, 94 x 76 mm
3905	Titan III Iso-Flur Cellulose Acetate, 60 x 76 mm
3906	Titan III Iso-Flur Cellulose Acetate, 94 x 76 mm
5805	Electra HR Buffer, 10 x 18 g

Titan III Iso-Vis membranes are used with Indolyl Blue reagent (Cat. No. 5102) or the Flur-Vis reagent set while Titan III Iso-Flur is used with ALP Flur reagent. Both are packaged 25 per box and available in two sizes. Electra HR Buffer is a tris-barbital/sodium barbital buffer used in the electrophoretic separation of ALP isoenzymes.