TITAN III Hemoglobins, Alkaline & Citrate Agar

Helena catalog

Titan® III and Titan IV® Hemoglobins
Cellulose Acetate and Citrate Agar

Numerous hemoglobin variants, many capable of producing severe hemolytic anemia and even death, have been identified. This has led to widespread implementation of neonatal screening programs. Counseling of individuals or marital partners, regarding the risk of having children with abnormal hemoglobins, is also common. Early detection of certain abnormal hemoglobins can alert parents to possible problems before onset of symptoms and allow physicians to better manage patients with hemoglobin variants.

Electrophoresis is generally considered the best method for separating and identifying abnormal hemoglobins. Via a two-step process, blood samples are initially screened using alkaline hemoglobin electrophoresis, followed by confirmation of abnormals by electrophoresis at an acid pH.

Helena hemoglobin procedures are widely used for hemoglobin screening programs. Many samples can be handled quickly, easily and cost-effectively. Interpretation is made by comparing the patient's hemoglobin pattern to a control sample.

Cellulose Acetate Assay Time: approximately 40 minutes.

Citrate Agar Assay Time: approximately 55 minutes.

Hemoglobin Controls

Cat. No.	Item
5328	AA2 Hemo Control, 1 x 1 mL
5329	ASA2 Hemo Control, 1 x 1 mL
5330	AFSA2 Hemo Control, 1 x 1 mL
5331	AFSC Hemo Control, 1 x 1 mL

Hemo Controls are stabilized liquids prepared from fresh-packed human red blood cells hemolyzed with Hemolysate Reagent. They are for qualitative and quantitative use in identification and quantitation of hemoglobins. AFSC Hemo Control is recommended for use with citrate agar.

Hemolysate Reagents

Cat. No.	Item
5125	Hemolysate Reagent, 1 x 250 mL
5127	Hemolysate Reagent w/o KCN, 1 x 250 mL
5128	Hemolysate Reagent (Powder), 1 x 1.8 g

This reagent hemolyzes red blood cells and stabilizes hemoglobin in one easy step. It eliminates many tedious, time-consuming steps in preparing hemolysates. The reagent contains 0.005 M EDTA with 0.07% potassium cyanide to assist in stabilizing the hemoglobin. The powdered reagent is dissolved in 1.0 liter deionized water.

Titan IV Citrate Agar and Citrate Buffer for Hemoglobins

Cat. No.	Item
2400	Titan IV Citrate Agar, 5/pkg
5121	Citrate Buffer, 10 x 15.5 g

The lower pH of the Titan IV Citrate Agar allows it to be used as a primary screening procedure for detection of HbS in newborns and as a confirmation of abnormal hemoglobins detected by alkaline electrophoresis. Up to 14 samples plus two controls can be run on each plate. Samples are easily applied with Zip Zone Applicator (Cat. No. 4080). Citrate Buffer is used as the chamber buffer for the citrate agar procedure.

Hemoglobin-Specific Stains

Cat. No.	Item
5036	o-Dianisidine, 1 x 2 g
5041	o-Tolidine, 1 x 2 g

o-Dianisidine is a sensitive, hemoglobin-specific stain used for staining hemoglobins separated on Titan IV Citrate Agar. An alternate stain for use with Titan IV Citrate Agar, o-Tolidine yields blue-colored hemoglobin bands. Both stains are powders that are dissolved in 1.0 liter methanol for use.

Titan III-H Cellulose Acetate and Supre-Heme Buffer for Hemoglobins

Cat. No.	Item
3021	Titan III-H Cellulose Acetate, 94 x 76 mm
3022	Titan III-H Cellulose Acetate, 60 x 76 mm
5802	Supre-Heme Buffer, 10 x 14.6 g

Titan III-H Cellulose Acetate membranes are for use in the alkaline hemoglobin electrophoresis. Membranes are available in two sizes and packaged 25 per box. Supre-Heme Buffer is a Tris-EDTA-Boric Acid buffer used in the alkaline hemoglobin procedure.

Complete the Cellulose Acetate and Citrate Agar Hemoglobin Procedures with these supplies: Ponceau S Stain (Cat. No. 5526), Clear Aid (Cat. No. 5005), and Titan IV Aligning Base (Cat. No. 4083).

Hemoglobin F Immunodiffusion Kit

Cat. No.	Item
9325	HbF QUIPlate Kit, 3 x 16 wells

The HbF QUIPlate Kit is for quantitation of Hemoglobin F by radial immunodiffusion. RID is much simpler and requires much less hands-on time than cumbersome alkali denaturation methods, which are also unreliable at low levels of HbF. The reference standards provided with the kit ensure accurate and precise quantitation from 0.5% to 10% HbF and are prepared from lyophilized hemoglobin. Samples with >10% HbF should be diluted with HbF Diluent. The crisp, well-defined precipitin rings can be read at 24 hours. Kit contains three 16-well plates, plus standards and graph paper for preparing a standard curve.

HbF QUIPlate Control and Diluent

Cat. No.	Item
9340	HbF QUIPlate Control, 1 x 0.2 mL
9341	HbF QUIPlate Diluent, 1 x 2.0 mL

HbF Control is a stabilized liquid prepared from human cord blood. It is used to verify plate/curve stability, allowing re-use of the standard curve for up to 28 days. HbF Diluent is prepared from purified human hemoglobin, providing a diluent completely free of HbF for diluting patient samples with >10% HbF.

Complete the QUIPlate procedure with: Dialamatic 1-10 mL Microdispenser (Cat. No. 6210).