TITAN III LD Isoenzymes

Helena catalog

Titan® III LD Isoenzymes
Lactate dehydrogenase (LD) is found in all body tissues. Normal human serum contains small amounts of LD due to normal tissue breakdown. Nonspecific increases of total LD in serum will occur following tissue damage. LD has been separated into five isoenzymes. Heart muscle contains LD1 and the liver and skeletal muscle contains LD5. The LD isoenzyme fractions are elevated when damage occurs to these tissues. A unique profile develops over time following damage to the heart, making it easy to distinguish between myocardial infarction and liver disease, congestive heart failure, shock, or skeletal muscle diseases.

LD Isoenzyme Assay Time: approximately 40 minutes.

LD Vis Isoenzyme Reagent

Cat. No.	Item
5909	LD Vis Isoenzyme Reagent, 10 x 3 mL

The LD Vis Isoenzyme Reagent develops isoenzyme bands for colorimetric detection. Quantitation by densitometry is accomplished by measuring the absorbance of a blue formazan, produced by reducing a tetrazolium salt. Reagent is reconstituted with deionized water.

CK/LD Isoenzyme Control

Cat. No.	Item
5134	CK/LD Isoenzyme Control

Control is manufactured from murine tissue extract and human serum, and is intended for use in the quantitative and/or qualitative determination of CK and LD isoenzymes by cellulose acetate and agarose electrophoresis. Three CK and five LD isoenzymes are present.

Titan III Cellulose Acetate and HR Buffer for LD Isoenzymes

Cat. No.	Item
3000	Titan III Iso-Vis Cellulose Acetate, 60 x 76 mm
3001	Titan III Iso-Vis Cellulose Acetate, 94 x 76 mm
3905	Titan III Iso-Flur Cellulose Acetate, 60 x 76 mm
3906	Titan III Iso-Flur Cellulose Acetate, 94 x 76 mm
5805	Electra HR Buffer, 10 x 18 g

Titan III Iso-Vis is used with LD Vis Reagent for visible LD isoenzyme detection. Titan III Iso-Flur is for use with fluorescent development methods. Membranes are available in two sizes and packaged 25 per box. The Electra HR Buffer is a tris-barbital/sodium barbital buffer used in separating CK and LD isoenzymes.