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Helena Laboratories Hemostasis
Other Hemostasis



Protein C Antigen Rocket PlatesProtein C Antigen Rocket Plates

Cat. No. Item
5357 Protein C Antigen Rocket Plates
  Protein C Rocket Plates, 5 x 12 wells/plate
  Rocket Antigen Report Form, 1 sheet
  Rocket Ruler, 1/pkg

The Protein C Rocket procedure is for quantitation of plasma Protein C Antigen by Laurell Rocket electrophoresis. The plate contains antiserum specific for Protein C. After electrophoresis, a rocket-shaped precipitin pattern forms along the axis of migration. The length of the rocket is proportional to antigen concentration.


 

Protein S Antigen Rocket PlatesProtein S Antigen Rocket Plates

Cat. No. Item
5359 Protein S Antigen Rocket Plates
  Protein S Rocket Plates, 5 x 12 wells/plate
  Rocket Antigen Report Form, 1 sheet
  Rocket Ruler, 1/pkg

The Protein S Rocket procedure is for quantitation of plasma Protein S antigen (either total or free) by Laurell Rocket electrophoresis. The plate contains antiserum specific for Protein S. After electrophoresis, a rocket-shaped precipitin pattern forms along the axis of migration. The length of the rocket is proportional to antigen concentration. Free Protein S can be assayed on the same plate as total Protein S by treating the sample with Free Protein S Reagent (Cat. No. 5363).

Free Protein S ReagentFree Protein S Reagent

Cat. No. Item
5363 Free Protein S Reagent, 5 x 2 mL

The reagent is a solution of PEG used to separate free Protein S from bound Protein S. For use with the Protein S Antigen Rocket Plates.

 

von Willebrand Factor Antigen (vWF:Ag) Rocket Platesvon Willebrand Factor Antigen (vWF:Ag) Rocket Plates

Cat. No. Item
5361 vWF:Ag Rocket Plates
  vWF:Ag Rocket Plates, 5 x 10 wells/plate
  Rocket Antigen Report Form, 1 sheet
  Rocket Ruler, 1/pkg


Accurate quantitative measurement of vWF:Ag in human plasma aids in differentiating von Willebrand Disease from Hemophilia A and in detecting carriers of Hemophilia A. Quantitation of vWF:Ag by Laurell rocket electro-immunodiffusion combines the specificity of immunochemistry with the speed of electrophoresis. The kit contains 5 plates with antiserum to human von Willebrand antigen incorporated into agarose.

 



Buffers for Rocket AssaysBuffers for Rocket Assays

Cat. No. Item
5358 Tris-Tricine Buffer, 10 x 14.1 g
5016 Electra B1 Buffer, 10/pkg

Tris-tricine Buffer is used in the Protein C and Protein S rocket electroimmunoassay methods. Electra B1 Buffer is used in the von Willebrand Factor Antigen rocket electroimmunoassay.




von Willebrand Factor Antigen (vWF:Ag) by ELISAvon Willebrand Factor Antigen (vWF:Ag) by ELISA

Cat. No. Item
5290 vWF:Ag ELISA Kit, 96 tests
  vWF:Ag Microwell Plate, 12 x 8-well strips
  Sample Diluent, 1 x 60 mL
  ELISA Reference Plasma, 3 x 0.5 mL
  vWF:Ag Conjugate Solution, 1 x 12 mL
  Substrate, 1 x 13 mL
  Stopping Solution, 1 x 15 mL
  Phosphate Buffered Saline Concentrate, 1 x 30 mL

This vWF:Ag assay uses an ELISA antibody capture sandwich technique to quantitate vWF:Ag in human plasma and is standardized against established reference preparations. Assay time is about an hour and final results are read at 450 nm. The key advantages of this new ELISA assay are its fast turnaround time and its ease of use. vWF:Ag is important in evaluating the pro-coagulant system, and von Willebrand Disease in particular.

Protein C Antigen by ELISAProtein C Antigen by ELISA

Cat. No. Item
5291 Protein C ELISA Kit, 96 tests
  Protein C Microwell Plate, 12 x 8-well strips
  Sample Diluent, 1 x 60 mL
  ELISA Reference Plasma, 3 x 0.5 mL
  Protein C Conjugate Solution, 1 x 12 mL
  Substrate Solution, 1 x 13 mL
  Stopping Solution, 1 x 15 mL
  Phosphate Buffered Saline, 1 x 30 mL

This Protein C assay uses an antibody capture sandwich technique to quantitate Protein C in human plasma. Quantitative deficiencies of Protein C are associated with an increased tendency for thrombosis. The Protein C ELISA assay can be completed in about 90 minutes and is read at 450 nm. Its fast turnaround time and ease of use offer advantages over other Protein C assays.

 

Free and Total Protein S Antigen by ELISAFree and Total Protein S Antigen by ELISA

Cat. No. Item
5292 Protein S (Free & Total) ELISA Kit, 96 tests
  Protein S Microwell Plate, 12 x 8-well strips
  Sample Diluent, 1 x  60 mL
  Free Protein S Reagent (PEG), 1 x  2 mL
  ELISA Reference Plasma, 3 x 0.5 mL
  Protein S Conjugate Solution, 1 x  12 mL
  Substrate Solution, 1 x  13 mL
  Stopping Solution, 1 x  15 mL
  Phosphate Buffered Saline, 1 x  30 mL

This 90-minute ELISA assay for quantitation of free and total Protein S in human plasma is easy to perform and offers a rapid turnaround time. The assay is read at 450 nm. To determine free Protein S, the physiologically active form, the sample is pretreated with PEG to precipitate the C4b-binding protein complex. Deficiencies of Protein S are associated with increased thrombosis.


Monoclonal Free Protein S Antigen by ELISAMonoclonal Free Protein S Antigen by ELISA

Cat. No. Item
5293 Monoclonal Free Protein S ELISA Kit, 96 tests
  Free Protein S Microwell Plate, 12 x 8-well strips
  Sample Diluent, 1 x 60 mL
  ELISA Reference Plasma, 3 x 0.5 mL
  Protein S Conjugate Solution, 1 x 12 mL
  Substrate Solution, 1 x 13 mL
  Stopping Solution, 1 x 13 mL
  Phosphate Buffered Saline, 1 x 30 mL

This assay uses a monoclonal antibody specific for Free Protein S in an ELISA format to measure Free Protein S directly without PEG precipitation. Incubation times for this procedure total 1 hour, allowing timely completion of testing. Results are read at 450 nm and calculated by comparison to an internationally recognized reference plasma.



Other Hemostasis

 

Protein C Antigen Rocket Plates

Protein S Antigen Rocket Plates

Free Protein S Reagent

von Willebrand Factor Antigen (vWF:Ag) Rocket Plates

Rocket Stain

Buffers for Rocket Stain

ELISA Assays